C calcium carbonate CACO3 - a white precipitate that forms in water lines, water heaters and boilers in hard water areas; also known as scale. In some igneous rocks, caverns can be formed by large gas bubbles.
Follow along as our Director of Biology, Dr. John Melville, walks through how to measure pressure changes as a plant takes up water into the stem. And today, I'm going to be showing you how to conduct a transpiration experiment or to measure transpiration from a plant.
This is a very popular lab that can be found in Biology with Vernier, Advanced Biology with Vernier, and also our Investigating Biology with Vernier lab book. Now, the most important thing you need to know to get this lab to work, is you need to know how to properly set it up.
So that's what I'm going to be focusing on. Now, one of the most important things you need to know is to get this lab to get to work properly, you really do need to use a woody stemmed plant, not a tomato or potato plant, but a woody Plants transpiration lab plant.
This is a fresh cut limb, small branch, from a Plants transpiration lab tree. The next thing you need to know is that you need to make a very fresh cut of the woody stem, and I like to use a razor blade.
And you want to cut that at an angle. Then you can just place it in some water and let it equilibrate and sit, and it should be fine.
Now, the reason why I say using a razor blade is because you can make an angled cut with a razor blade, and you're not going to crush the limb.
If you use a scissors, you're going to crush it, and that can be a real problem. The other thing that you're going to need to do this lab is a gas pressure sensor, a syringe, some tubing, a little stopcock, some hose clamps, and then two utility clamps, a stand, and in this case, I'm using a LabQuest 2.
Now, all of these smaller items here, the syringe, and the tubing, and the luer lock, and the hose clamps with the stopcock, they all come with the gas pressure sensor. You can also buy them separately as the gas pressure sensor accessory kit. So you should have all of these items laying around if you have a gas pressure sensor.
The next thing that I'm going to show you is really important, and that's actually how to place the stem into the tubing. So first thing that we're going to do, is we're going to fill the tubing with some water.
So I'm going to remove one of these little pieces from the tubing, and then I'm going to fill it with water by placing the syringe into the other end of the tubing. And I'm going to place the stopcock on the other end of the tubing. I'm just going to make sure it's open, and then I'm going to press water through the tubing until it flows out the other end.
And I want to make sure there are no air bubbles in it, so I'm just going to gently press through until water begins to flow through it.
It's dripping through there, and then you could see a nice little bead of water on the top. And then I'm going to close the lock.
And then now what I'm going to do is I'm going to gently press on the syringe to make sure that there is no air bubble in the tip here either.
So, as I begin to pull off, I'm going to just gently press down on the syringe so that I make sure that this fills up with some water.
And then just gently twist off. You might get a little bit wet.* Transitioned from the AP Biology Lab Manual () S Investigation 11 Bio_T_Lab11_01 Water lost by transpiration Water absorbed by root hairs Suction pressure Capillarity Figure 1.
Transpiration Model several questions emerge about the process of transpiration in plants, including the following: • Do all plants have stomata? Is there. Different plants also vary in the rate of transpiration and in the regulation of stomata openings. (Copy the big idea, enduring understandings and learning objective codes in your lab notebook) Big Idea 4.
Transpiration Introduction Most of the water a plant absorbs is not used for a plant’s daily functioning. It is instead lost through transpiration, the evaporation of water through the leaf surface and stomata, and through guttation, which is the loss of water from the vascular tissues in the margins of leaves.
There are three levels . We would like to show you a description here but the site won’t allow us. The plants with the larger leaves (zebra plant, weeping fig) the more transpiration. -I also think the location and the amount of moisture in the water has a major influence on how much water is available in the plant to transpire.
Laboratory: Water Movement in Vascular Plants I. Transpiration and the Vascular System About 1% of the water taken up by plants is used for metabolic functions such as photosynthesis.
A plant's root hairs are located near the growing tip of the root. Root hairs form as.